Experimental

Experimental#

Currently in development, right now as an outline

Tissue#

Tissues are quite heterogeneous, and you must know your tissue to best adapt the workflow to it.

  • Adhesion to membrane

  • Autofluorescence

  • Cell size

  • Cell density

  • Tissue thickness

Membrane Slides#

Laser microdissection of tissue cannot be done on glass slides, they stick to well. Therefore we collect tissue from slides that have a membrane. The properties of the membrane vary, and you should consider which slide to use.

Leica offers a variety of slides:

  • Frame Slide

  • Membrane Slides

  • PPS

Preparation for laser microdissection#

Table with main properties. Consider showing what each project used.

Imaging modalities#

You can image with whatever technology suits your project best. In general most people use:

  • H&E: Quick, simple, cheap, and pathology friendly

  • IHC: Targeted with antibody, does not require fluoresence

  • IF: Targeted with antibodies, a single round can have up to 5 stains.

  • mIF: IF with manual handling between cycles

Hematoxylin and Eosin#

Immunohistochemistry#

Immunofluorescence#

Note: Here you should consider creating calibration points by etching the membrane with the LMD.

Multiplex Immunofluorescence#

Note: Here you should consider creating calibration points by etching the membrane with the LMD.

Microscopes and critical settings#

Magnification#

Binning#

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